Enable 2FA for Users

While it's still not possible to force the configuration of a 2FA at the user level without the Devolutions Server , a few options have been added in Remote Desktop Manager that could suit your needs.

These features are only available when using an Advanced Data Source

You cannot configure 2FA for users. Once activated, each user will need to configure it manually in File - DataSources For more information about configuration consult this topic: 2-Factor Authentication

Force application security with Windows credentials

Under Administration - System Settings - Applications - Security - Force application security with Windows credentials

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Force application security with TOTP (Authenticator)

Under Administration - System Settings - Applications - Security - Force application security with TOTP (Authenticator)

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Force data source 2-factor configuration

Under Administration - System Settings - Security Settings - Security - Force data source 2-factor configuration

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Force with Group Policies (GPO)

We also have GPOs that could help you achieve your goal. An example in this case is the Force multiple-factor authentication on the application login

%Root%\SOFTWARE\Policies\Devolutions\RemoteDesktopManager\ForceApplicationMFA

For more information about configuration, consult this topic How to Apply Policies